The finalists include Seloy Live, a Finnish firm specialising in transforming shop windows into interactive displays; Oriient, an Israeli company that guides consumers around stores via a custom smartphone app; and Memomi, a US business that enables consumers to try on clothes virtually using a detailed image profile.
Three British startups are also among the final six, with Ruuby enabling customers to book beauty treatments at home; Makers Café enabling the 3D printing of personalised products; and Lettus Grow, which utilises retail spaces as soil-free farms.
Speaking to City AM, John Lewis’ customer experience director Peter Cross said: "As far as John Lewis is concerned, the sweet spot is the place where human meets digital technology.
"We believe the dynamic new businesses selected for further discussions with JLab will help us continue to stretch, shape and deliver together for our customers in the future."
The six were chosen by a panel of judges who heard submissions from 11 startups earlier this month, with each now entering detailed discussions around a potential partnership.
Past winners of the John Lewis startup programme include internet of things home security device maker Peeple.